BBa_K112318

BBa_K112318 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112318

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{< bolA promoter>} in BBb format

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K112318_sequence (Version 1)

Description

bolA promoter is a promoter where levels of expression are inversely proportional to the growth rate. To read more, go to following link http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=402084&blobtype=pdf This part is actually in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0017/al0018 on MG1655     (467bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112318



al0017	Forward Biobricking of bolA promoter

CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc

al0018	Reverse Biobricking of bolA promoter

CGttaGGATCCcttctatccgctcacgtatc

Source

MG1655

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112318/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter
• //regulation/multiple
• //regulation/negative
• //rnap/prokaryote/ecoli/sigma70
• //rnap/prokaryote/ecoli/sigmas
• iGEM Parts Registry