Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yuta Otsuka
Date created: 2013-09-10 11:00:00
Date modified: 2015-05-08 01:09:17
amilGFP (+LVA), yellow reporter protein with degradation tag
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1124000_sequence (Version 1) |
Description
amilGFP (+LVA)yellow reporter protein with degradation tag.
The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging . It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki.
This is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013).
amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family.
Excitation maximum : 503 nm
Emission maximum : 512 nm
Quantum yield : 0.67
Molar Extinction : 75200
LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus.
References
Andersen, J. B., Sternberg, C., Poulsen, L. K., Bj??rn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.
Notes
LVA tag(11 aa) was added by PCR. As in the previous study(1). Additional 2 aa (Stu1 restriction site) was also added, which may not be necessary for unstabilization of the protein.RPAANDENYALVA
double stop codon
[1]Andersen, J. B., Sternberg, C., Poulsen, L. K., Bj??rn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.