BBa_K1126001

BBa_K1126001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1126001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kanji Nakagawa
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:09:18

TetR aptamer 12_1P



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K1126001_sequence (Version 1)

Description

This part is an RNA aptamer that induces TetR-controlled gene expression in Escherichia coli when expressed in the cell. The aptamer was found by a combined approach of in vitro selection for TetR binding and in vivo screening for TetR induction. The smallest active aptamer folds into a stem-loop with an internal loop interrupting the stem. The TetR-inducing activity of the aptamer directly correlates with its stability and the best construct is as efficient as the natural inducer tetracycline. Because of its small size, high induction efficiency, and the stability of the TetR aptamer under in vivo conditions, it is well suited to be an alternative RNA-based inducer of TetR-controlled gene expression.

Notes

Nothing

Source

http://www.sciencedirect.com/science/article/pii/S1074552109000064

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1126001/1