Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: York iGEM 2013
Date created: 2013-09-04 11:00:00
Date modified: 2015-05-08 01:09:18
Gold sensing device (PgolTS+LacZ+GolS)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1127008_sequence (Version 1) |
Description
This is the device we use in our project so that the cell could recognize the presence of gold in it's environment.GolS protein acts as a positive transcriptional regulator on PgolTS once it binds to gold.
Part was designed so that you can detect and quantify the activity of the promoter via Beta-galactosidase assay. For convenience the ORF of LacZ is covered by restriction sites of SacII and SanDI which allows the cell to respond to gold by expressing something to meet your needs.
Notes
N/ASource
The part itself was synthesized fully [i]de novo[/i].DNA sequence for PgolTS comes from Salmonela enterica as does the amino acid sequence for GolS (DNA sequence is different however).
| Sequence Annotation | Location | Component / Role(s) |
| PgolTS SacII rbs lacZ-alpha SandI GFP wat? rbs GolS | 1,39 40,45 46,57 78,263 265,271 64,77 272,283 290,754 | promoter feature/promoter feature/dna sequence_feature ribosome_entry_site feature/rbs feature/cds CDS sequence_feature feature/dna sequence_feature feature/misc feature/rbs ribosome_entry_site feature/cds CDS |