Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:18
hns promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K112701_sequence (Version 1) |
Description
Phns in BBb1 format.This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Source
Construction of [] Basic Part K112701
PCR dv001/dv006 on Phns from E. coli K12 MG1655 (~342 bp, gp = A)
PCR dv005/dv003 on Phns from E. coli K12 MG1655 (225 bp, gp = B)
PCR dv004/dv002 on Phns from E. coli K12 MG1655 (~149 bp, gp = C)
PCR dv001/dv003 on A+B (355 bp, gp = D)
PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+697)
Product is pBca1256-K112701
dv001 Forward Biobricking of [] CgATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC
dv002 Reverse Biobricking of [] cagtcggatccGCACGAAGAGTACGGATG
dv003 Removing the 2nd EcoRI site in [] (R) GCCAGGAATGTAAGGgATTCAAAATTGTTC
dv004 Removing the 2ND EcoRI site in [] (F) GAACAATTTTGAATcCCTTACATTCCTGGC
dv005 Removing the FIRST EcoRI site in [] (F)CGCTTAATAGGGgATTCTCGTAAACAC
dv006 Removing the FIRST EcoRI site in [] (R)GTGTTTACGAGAATcCCCTATTAAGCG