S

BBa_K112705 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112705

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:18

S affinity tag

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_K112705_sequence (Version 1)

Description

This S-tag can be added between two protein parts.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

Construction of S-tag> basic part K112705



Wobble PCR dv013/dv014                (47 bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+47, L)

Product is pBca1256-K112705



dv013	Forward Biobricking of S-Tag>

cgataGAATTCatgAGATCTatgaaagaaaccgctgctgctaaattcg

dv014   Reverse Biobricking of S-Tag>

cgttaGGATCCgctgtccatgtgctggcgttcgaatttagcagcagcgg

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112705/1

Member of these Collections

• //proteindomain/affinity
• //proteindomain/head
• iGEM Parts Registry