BBa_K112708

BBa_K112708 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112708
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:18

PfhuA



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_K112708_sequence (Version 1)

    Description

    The fhuA promoter.

    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Source


    
Construction of  basic part K112708
    

    
PCR of dv019/dv020 on   MG1655          (225bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256                        (EcoRI/BamHI, 2472+225, L)
    
Product is pBca1256-K112708
    


    
dv019 Forward biobricking of       
    
cgataGAATTCatgAGATCTgctaagcgtgaaataccggatgg
    

    
dv020 Reverse biobricking of 
    
cgttaGGATCCactctgatgtaaagtgaatgataacg


    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112708/1
     

    Attachments

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