BBa_K112711

BBa_K112711 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112711

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:18

rbs.spvR!

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K112711_sequence (Version 1)

Description

The spv repressor, with an rbs and stop codon.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

Construction of rbs.SpvR! basic part K112711



PCR of rbs.SpvR! al0027 and al0028   (933bp, EcoRI, BamHI, DpnI)

Sub into pBca1256                        (EcoRI/BamHI, 2472+933bp, L)

Product is pBca1256-K112711





al0027 Forward Biobricking of rbs.spvR! CCATAGAATTCatgAGATCTAAAAAAGGAGATATTATGGATTTCTTG



al0028 Reverse Biobricking of rbs.spvR! CGttaGGATCCaggtcagaaggtggactg

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112711/1

Member of these Collections

• iGEM Parts Registry