BBa_K112808

BBa_K112808 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112808

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jin Huh

Date created: 2008-10-31 12:00:00

Date modified: 2015-05-08 01:09:19

Enterobacteria phage T4 Lysis Device - no promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K112808_sequence (Version 1)

Description

This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Various promoters can be installed with this device to control the lysis of bacteria. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.

Notes

[Pcon].[Antiholin].[terminator] was assembled first, and then assembled with [T4 holin].[terminator]; Constitutive expression of antiholin is required before assembling holin.

Source

Pubmed Accession Number: NC_000866
Enterobacteria phage T4

Components

Access	Instance	Definition
public	BBa_K112805	BBa_K112805
public	BBa_K112806	BBa_K112806
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_J23116	BBa_J23116
public	BBa_K112807	BBa_K112807
public	BBa_B0010	BBa_B0010

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K112805	1,676	BBa_K112805
BBa_K112806	685,1198	BBa_K112806
BBa_B0010	1207,1286	BBa_B0010
BBa_B0012	1295,1335	BBa_B0012
BBa_J23116	1344,1378	BBa_J23116
BBa_K112807	1387,1697	BBa_K112807
BBa_B0010	1706,1785	BBa_B0010

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112808/1

Member of these Collections

• //function/celldeath
• iGEM Parts Registry