Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: UBC iGEM 2013
Date created: 2013-08-29 11:00:00
Date modified: 2015-05-08 01:09:19
Complete caffeine synthesis pathway under pTET constitutive promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1129019_sequence (Version 1) |
Description
We combined the three caffeine synthesis genes in the Registry by standard assembly into a pSB1C3 vector. This vector had a constitutive promoter, BBa_J23118, and a bacterial consensus sequence cloned into it previously, so that the completed construct would allow constitutive expression of the entire caffeine biosynthesis pathway when the substrate xanthosine was present.Notes
We have yet to test for expression, and we may find that codon optimization is needed.Source
The constitutive promoter we used was from the 2012 Distribution Kit, part BBa_J23118. The caffeine biosynthesis genes we assembled were from the 2013 Distribution Kit and were submitted by the 2012 TU Munich team: parts BBa_K801070, BBa_K801071, BBa_K801072.| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_J23118 BBa_B0034 BBa_K801070 BBa_B0015 BBa_J23118 BBa_B0034 BBa_K801071 BBa_B0015 BBa_J23118 BBa_B0034 BBa_K801072 BBa_B0015 | BBa_J23118 BBa_B0034 BBa_K801070 BBa_B0015 BBa_J23118 BBa_B0034 BBa_K801071 BBa_B0015 BBa_J23118 BBa_B0034 BBa_K801072 BBa_B0015 |