Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: WU Jingjing, Wang Jinyu
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:09:20
pT7->tetR operator->GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K113004_sequence (Version 1) |
Description
tetR operator is inserted between T7 promoter and RBS in BBa_I719015, which is a T7 promoter-regulated GFP sequence. Thus tetR can repress the expression of GFP once the concentration of tetR reaches certain level.Notes
For repressor test (right side).----HKUSTers/2008Source
T7 promoter is from BBa_I719015.tetR operator is the same as BBa_R0040.
RBS + GFP + T7 terminator is from BBa_I719015.
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I719005 tetR operator BBa_B0034 BBa_E0040 GFP protein BBa_B0010 BBa_B0012 | 1,23 27,73 82,93 100,819 100,819 828,907 916,956 | feature/BioBrick engineered_region operator feature/operator feature/BioBrick engineered_region engineered_region feature/BioBrick CDS feature/protein engineered_region feature/BioBrick engineered_region feature/BioBrick |