Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wu Jingjing, Wang Jinyu
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:09:20
more overlapping T7 promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K113011_sequence (Version 1) |
Description
Two T7 promoters are reversely overlapping in five base pairs, with the third one mutated from A to G.Notes
It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.Source
The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter T7 promoter | 4,21 17,34 | feature/promoter promoter feature/promoter promoter |