Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wu Jingjing, Wang Jinyu
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:09:20
weaken overlapping T7 promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K113012_sequence (Version 1) |
Description
Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.Notes
It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.Source
The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter T7 promoter | 4,21 20,37 | feature/promoter promoter promoter feature/promoter |