BBa_K1131000

BBa_K1131000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1131000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ramya Prathuri

Date created: 2013-08-18 11:00:00

Date modified: 2015-05-08 01:09:20

Flavin-containing monooxygenase (FMO)
M. aminisulfidivorans

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1131000_sequence (Version 1)

Description

This Flavin-containing monooxygenase (FMO) from M. aminisulfidivorans can be expressed in many strains of E. Coli to produce indigo dye. In the presence of indole and oxygen, FMO can catalyze the addition of a hydroxyl group to indole generating the intermediate indoxyl. Indoxyl then naturally oxidizes to generate indigo which, due to its hydrophobicity, crashes out of solution. The part submitted is the ORF of FMO only.

Notes

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Source

This part comes from the M. aminisulfidivorans genome. We obtained it however from Han et. al 2011 - (Bio-indigo production in two different fermentation systems using recombinant Escherichia coli cells harboring a flavin-containing monooxygenase gene (fmo)).

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1131000/1

Member of these Collections

• iGEM Parts Registry