| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1131005_sequence (Version 1)
|
Description
This is the mutant version of oleD glucosyltransferase part BBa_K1131004. Mutations identified by Thorston et. al. 2010 at P67T, S132F, and A242V were introduced to oleD generating the 'ASP' version of the protein. OleD 'ASP' is reported to have a much larger set of substrates it can glucosylate compared to the non mutated oleD.
Notes
The part has an additional 15 amino acids at the C-terminus and has the same sequence as uniprot Q53685, except with the 3 mutations described above.
Source
The source of this part was a plasmid donated from the Anderson lab and subsequently modified. The 'ASP' mutation comes from Thorston et. al. 2010
