Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Toulouse
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:20
R0 riboregulator switch with pTET and pLuxRCI
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1132005_sequence (Version 1) |
Description
The R0 riboregulator sequence is designed based on the publication of Callura JM (http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/22454498). The principle is to introduce short regulatory sequences that create RNA secondary structures to better control protein expression. The basic design is reported in the following scheme (red hexagonal boxes, terminators; green oval box, rbs; blue and red rectangles, riboregulator sequences; arrows, promoters). Promoters P1 and P2 are controlling the expression of a single gene X (not present in the biobrick). Two terminators are placed between P1 and P2.Notes
The R0 riboregulator sequence is designed based on the publication of Callura JM (http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/22454498). The principle is to introduce short regulatory sequences that create RNA secondary structures to better control protein expression. The basic design is reported in the following scheme (red hexagonal boxes, terminators; green oval box, rbs; blue and red rectangles, riboregulator sequences; arrows, promoters). Promoters P1 and P2 are controlling the expression of a single gene X (not present in the biobrick). Two terminators are placed between P1 and P2.Source
later| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 pTET P1 riboregulation sequence BBa_B0015-Double terminator Cla I site BBa_R0065 - pLuxRCI Bam HI site P2 riboregulation sequence | 1,54 55,119 120,286 287,292 293,389 390,395 396,435 | feature/promoter promoter sequence_feature feature/misc stop_codon feature/stop non_covalent_binding_site feature/binding promoter feature/promoter feature/binding non_covalent_binding_site feature/misc sequence_feature |