Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Toulouse
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:20
R4 riboregulator switch with with pTET and pOmpC
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1132008_sequence (Version 1) |
Description
The R4 riboregulator sequence is designed based on the publication of Callura JM (http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/22454498). The principle is to introduce short regulatory sequences that create RNA secondary structures to better control protein expression. The basic design is reported in the following scheme (red hexagonal boxes, terminators; green oval box, rbs; blue and red rectangles, riboregulator sequences; arrows, promoters). Promoters P1 and P2 are controlling the expression of a single gene X (not present in the biobrick). Two terminators are placed between P1 and P2.Notes
The R4 riboregulator sequence is designed based on the publication of Callura JM (http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/22454498). The principle is to introduce short regulatory sequences that create RNA secondary structures to better control protein expression. The basic design is reported in the following scheme (red hexagonal boxes, terminators; green oval box, rbs; blue and red rectangles, riboregulator sequences; arrows, promoters). Promoters P1 and P2 are controlling the expression of a single gene X (not present in the biobrick). Two terminators are placed between P1 and P2.Source
later| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 - pTET P1 riboregulation sequence BBa_B0015 - Double terminator Cla I site BBa_R0082 - pOmpR Bam HI site P2 riboregulation sequence | 1,54 55,119 120,286 287,292 293,400 401,406 407,446 | feature/promoter promoter sequence_feature feature/misc feature/stop stop_codon non_covalent_binding_site feature/binding promoter feature/promoter feature/binding non_covalent_binding_site sequence_feature feature/misc |