Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cl??mence Mesnage
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:21
R1-pLac riboregulator switch with with pTET and pLac
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1132042_sequence (Version 1) |
Description
The R4 riboregulator sequence is designed based on the publication of Callura JM (http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/22454498). The principle is to introduce short regulatory sequences that create RNA secondary structures to better control protein expression. The basic design is reported in the following scheme (red hexagonal boxes, terminators; green oval box, rbs; blue and red rectangles, riboregulator sequences; arrows, promoters). Promoters P1 and P2 are controlling the expression of a single gene X (not present in the biobrick). Two terminators are placed between P1 and P2.Notes
The R4 riboregulator sequence is designed based on the publication of Callura JM (http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/22454498). The principle is to introduce short regulatory sequences that create RNA secondary structures to better control protein expression. The basic design is reported in the following scheme (red hexagonal boxes, terminators; green oval box, rbs; blue and red rectangles, riboregulator sequences; arrows, promoters). Promoters P1 and P2 are controlling the expression of a single gene X (not present in the biobrick). Two terminators are placed between P1 and P2.Source
later| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 - pTET P1 riboregulation sequence BBa_B0015 - Double terminator Cla I site BBa_R0011 - pLac Bam HI site P2 riboregulation sequence | 1,54 55,119 120,286 287,292 293,347 348,353 354,393 | feature/promoter promoter feature/misc sequence_feature feature/stop stop_codon feature/binding non_covalent_binding_site promoter feature/promoter non_covalent_binding_site feature/binding sequence_feature feature/misc |