Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Deyell
Date created: 2013-10-03 11:00:00
Date modified: 2015-05-08 01:09:21
pSB10K1
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K1137005_sequence (Version 1) |
Description
pSB10K1 ??? contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColA replicon with a copy number of 20 ??? 40 per cell. The cat gene has been replaced with kan cassette conveying kanamycin resistance.Notes
Designed to be functional with pSB9S1, pSB3C7, and pSB6A3Source
pSB1C3 is the template with the ori and resistance replaced with those from pCOLA| Sequence Annotation | Location | Component / Role(s) |
| Prefix mRFP Suffix ColA Terminator Terminator Kan Terminator Terminator | 1,20 248,925 1092,1111 1278,1913 1137,1205 1226,1266 2023,2838 3078,3149 3170,3247 | feature/BioBrick engineered_region CDS feature/cds engineered_region feature/BioBrick sequence_feature feature/misc stem_loop feature/stem_loop stem_loop feature/stem_loop CDS feature/cds feature/stem_loop stem_loop feature/stem_loop stem_loop |