Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Deyell
Date created: 2013-10-03 11:00:00
Date modified: 2015-05-08 01:09:21
pSB6A3
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K1137006_sequence (Version 1) |
Description
pSB6A3 - contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with ColE1 replicon derived from pBR322 with a copy number of approximately 40 per cell. The cat gene has been replaced with ampR cassette conveying ampicillin resistance.Notes
Designed to be stable with pSB9S1, pSB10K1, and pSB3C7Source
Original sequence used was pSB1C3, replacing the ori and resistance cassette with those from pET| Sequence Annotation | Location | Component / Role(s) |
| f1 ori ampR ColE1 Terminator Prefix mRFP Terminator Terminator | 65,512 638,1498 1593,2323 3502,3542 3544,3564 3792,4469 4636,4655 4656,4725 | sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc feature/stem_loop stem_loop feature/BioBrick engineered_region CDS feature/cds engineered_region feature/BioBrick feature/stem_loop stem_loop |