Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicolas Koutsoubelis, Anne Loechner
Date created: 2013-10-03 11:00:00
Date modified: 2015-05-08 01:09:21
gRNA anti KAN
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1137012_sequence (Version 1) |
Description
This rather simple part contains the UG6 promoter that controls the gRNA anti KAN cassette. This cassette consists of a seed sequence of the Kanamycin resistance gene (Aminoglycoside N6???-acetyltransferase) and the gRNA scaffold. This part is ready to use and can generate together with the CAS9 a double strand break in the kanamycin resistance gene. By definition, the gRNA is hybrid RNA out of the crRNA and tracrRNA. The gRNA is necessary to guide the CAS9 to the specific site and to cause there a double strand break. The design of the gRNA is taken from the DiCarlo et al. 2013 paper.Notes
-Source
This part was synthesized by IDT, the design is derived from the paper below.James E. DiCarlo, Julie E. Norville, Prashant Mali, Xavier Rios, John Aach and George M. Church (2013): Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Research, 2013, 1???8.