Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Janssel Reyes del Castillo
Date created: 2013-08-28 11:00:00
Date modified: 2015-05-08 01:09:22
RBS + TetR (LVA)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1140000_sequence (Version 1) |
Description
This synthesised construction has a gene that codes for the red fluorescent protein (RFP) variant that has an LVA degradation tag. The LVA tag that is in frame with the coding sequence of the gene, is actually a derivation of the C-terminal AANDENYALAA tagging sequence, which makes proteins susceptible to degradation by the ClpX and ClpA proteases.This gene is under the regulation of a pTetR promoter The coding sequence of TetR was derived from part BBa_C0040. TetR is a member of a family of transcriptional repressors present in gram-positive, alpha-,beta-, and gamma-proteobacteria, cyanobacteria and archea.
Its follows by a thermo-switch/ribosome binding site (RBS) that should allow for translation only at temperatures since 37??C. The RBS sequence is derived from part BBa_B0034, which features in the 2010 study by Elowtiz and Leibler. This RBS is defined as the standard for RBS activity and is assigned an efficiency of 1.0. The Terminator