BBa_K1140006

BBa_K1140006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1140006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Janssel Reyes del Castillo
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:09:23

pTet + 37 oC RNA thermometer + mCherry (LVA)



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1140006_sequence (Version 1)

Description

This part contains a promoter regulated by TetR protein. The Transcription is regulated by a 37 oC riboswitch. The protein codified is a RFP with a LVA tag.

Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. Promoter pTet. Based on a fusion of a RBS and a Riboswitch. RFP with a LVA tag (Further information coming soon).

Sequence Annotation Location Component / Role(s)
pTet
U6 RNA thermometer
mCherry+LVA
t7 terminator
1,54
63,121
122,835
844,887
feature/promoter promoter
stem_loop feature/stem_loop
feature/cds CDS
stem_loop feature/stem_loop
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1140006/1