BBa_K1140006

BBa_K1140006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1140006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Janssel Reyes del Castillo

Date created: 2013-09-12 11:00:00

Date modified: 2015-05-08 01:09:23

pTet + 37 oC RNA thermometer + mCherry (LVA)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1140006_sequence (Version 1)

Description

This part contains a promoter regulated by TetR protein. The Transcription is regulated by a 37 oC riboswitch. The protein codified is a RFP with a LVA tag.

Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. Promoter pTet. Based on a fusion of a RBS and a Riboswitch. RFP with a LVA tag (Further information coming soon).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pTet	1,54	feature/promoter, promoter
U6 RNA thermometer	63,121	stem_loop, feature/stem_loop
mCherry+LVA	122,835	feature/cds, CDS
t7 terminator	844,887	stem_loop, feature/stem_loop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1140006/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //function/reporter/fluorescence
• iGEM Parts Registry