Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg 2013
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:27
dCas9
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1150000_sequence (Version 1) |
Description
The human codon optimized Cas9 from the organism Streptococcus pyogenes was standardized (RFC 25). Additionally we mutated the Nickase function. In the end we engineered a DNA binding protein, that can be fused with different effectors in order to regulate gene expression.Notes
Restriction sites were deleted by primer overhangs. Parts were fused together by fusion PCR. The nickase function was mutated in order to abbolish the demolition of DNA.Source
Streptococcus pyogenes, Zhang lab| Sequence Annotation | Location | Component / Role(s) |
| dCas9 Nickaseless mutation | 1,4101 2515,2517 | CDS feature/cds sequence_alteration feature/mutation |