BBa_K115001

BBa_K115001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K115001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: O.M.J.A. Stassen
Date created: 2008-07-14 11:00:00
Date modified: 2015-05-08 01:09:27

RNA thermometer (ROSE)



Types
DnaRegion

Roles
RNA

mature_transcript_region

Sequences BBa_K115001_sequence (Version 1)

Description

This part is designed as a temperature inducible RBS. Only designed so far.

Notes

The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.

Source

This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).

Sequence Annotation Location Component / Role(s)
SD
Biobrick-Scar-adaptation
Predicted Stem Loop
Predicted Stem Loop
Predicted Stem Loop
Predicted Stem Loop extending to startcodon
90,95
74,79
1,15
16,36
39,71
72,96
ribosome_entry_site feature/rbs
sequence_alteration feature/mutation
stem_loop feature/stem_loop
stem_loop feature/stem_loop
stem_loop feature/stem_loop
feature/stem_loop stem_loop
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K115001/1