Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: O.M.J.A. Stassen
Date created: 2008-07-14 11:00:00
Date modified: 2015-05-08 01:09:27
RNA thermometer (ROSE)
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K115001_sequence (Version 1) |
Description
This part is designed as a temperature inducible RBS. Only designed so far.Notes
The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.Source
This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).| Sequence Annotation | Location | Component / Role(s) |
| SD Biobrick-Scar-adaptation Predicted Stem Loop Predicted Stem Loop Predicted Stem Loop Predicted Stem Loop extending to startcodon | 90,95 74,79 1,15 16,36 39,71 72,96 | ribosome_entry_site feature/rbs sequence_alteration feature/mutation stem_loop feature/stem_loop stem_loop feature/stem_loop stem_loop feature/stem_loop feature/stem_loop stem_loop |