| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1151000_sequence (Version 1)
|
Description
The HpNikR protein is a pleiotropic regulator from Helicobacter pylori. In presence of nickel it can acts as an activator or a repressor depending of the specific promoter that contains its operator sites. Each monomer consists in 133aa for a molecular weight of approximately 17.2 kDa. Apo-HpNikR is a tetramer able to bind up to six Ni2+ ions and then a conformational change allows it to activate or repress trascription.
Notes
It was inserted in a pET-15b plasmid vector, in order to have a better storage.
Source
It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.
