BBa_K1151001

BBa_K1151001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1151001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Davide Magr??
Date created: 2013-09-20 11:00:00
Date modified: 2015-05-08 01:09:28

Histidine-rich metal-binding protein



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1151001_sequence (Version 1)

Description

So called for emphasize its origins in Helicobacter pylori and its avidity for nickel. Consisting of 60 aminoacids, the protein is rich in histidine (28 residues, 46.7 %) and contains short repeating motifs, it exists as an equilibration of multimeric forms in solution, with 20-mers (approx. 136 kDa) being the predominant species. Hpn can bind tightly and reversibly up to five Ni2+ ions per each monomer of 7 kDa in a pH-dependent manner (pH 7.4 ). In H. pylori play an important role in storing nickel required to the survival of the bacterium.

Notes

The gene is designed on H.pylori genomic sequence and amplified by PCR

Source

synthesized by IDT (as gblock)

igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1151001/1