BBa_K1152009

BBa_K1152009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1152009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis
Date created: 2013-09-18 11:00:00
Date modified: 2015-05-08 01:09:29

Sfp 4'-Phosphopanthetheinyl-transferase



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1152009_sequence (Version 1)

    Description

    sfp encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes.

    Notes

    We cloned sfp in a pSB3K3-derived plasmid with a lac promoter and the RBS BBa_B0029.

    Source

    sfp originally comes from Bacillus subtilis strain 168. We amplfied it from E. coli BAP1 which carries the sfp gene.

    Sequence Annotation Location Component / Role(s)
    sfp cds
    		1,678
    		feature/cds CDS

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1152009/1
     

    Attachments

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