Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Solenne Ithurbide, Abdou, Ana??s, Zhu, Xavier, Nadia
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:09:30
Promoter of narK activated by FNR in anaerobic condition
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1155006_sequence (Version 1) |
Description
Promoter of gene nirB from E. coli. Activated by FNR in anaerobic conditions and inactivated in aerobic conditions.The promoter of the gene narK is regulated by FNR. It's a class II promoter with two FNR boxes. (Scott et al, FEBS letter, 2003)
Aerobic conditions: +O2 --> FNR inactivated --> Promoter non activated /
Anaerobic conditions: - O2 --> FNR activated --> Promoter activated
P(narK) is predicted to be highly induced under anaerobic conditions :48-64 fold. (Lolesnikow et al, J. bacteriol 1992 / Griffiths et al, Arch. Microbiol. 1987 / Green et al, Mol. Microbiol, 1998)
Notes
We conserved all the promoter region until the last nucleotide before narK ORF and approximately 100 bp before FNR boxesSource
Escherichia coli K-12 substr. MG1655, web site EcoCychttp://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10642
| Sequence Annotation | Location | Component / Role(s) |
| FNR box I ( ttgat) FNR box II ( atcaa) -10 ( taaggt) -35 ( tgcct) | 119,123 128,132 154,159 134,139 | feature/operator operator feature/operator operator operator feature/operator operator feature/operator |