BBa_K1156000

BBa_K1156000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1156000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haylee Bachman
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:30

RBS_T7_Pet(mCherry)



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1156000_sequence (Version 1)

Description

This is a composite part of an autodisplay technology translocating a fluorescent protein (mCherry). This part includes an T7 promoter with a lacI operator as well as an RBS(AGGA). The fluorescent protein will cleave post-translationally. This part can be used to quantify the surface density and activity of autodisplay technology. PET is a beta-barrel autodisplay technology native to E.coli. mCherry is a derivative of RFP.

Notes

To comply with BioBrick standard 10, Phe-76 was re-optimized to remove a EcoRI site within the part. A T7 promoter with a lacI operator was added with the RBS (AGGA) to the PET_mCherry. A standard assembly 10 prefix and suffix was then added.

Source

A sequence optimized for E. coli for a PET beta barrel auto-transporter expressing mCherry was obtained from Sevastynovich et al."A generalised module for the selective extracellular accumulation of recombinant proteins." Microbial Cell Factories. 2012. The sequence was assembled from synthesized 500bp blocks through IDTdna "Gblock" synthesis.

Sequence Annotation Location Component / Role(s)
Signal Peptide
mCherry
Autochaperone
Translocator Domain
Start
Stop
RBS
119,274
317,1024
1235,1546
1547,2458
119,121
2459,2466
9,12
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
sequence_feature feature/misc
start_codon feature/start
feature/stop stop_codon
ribosome_entry_site feature/rbs
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1156000/1