Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haylee Bachman
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:30
RBS_T7_Pet(mCherry)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1156000_sequence (Version 1) |
Description
This is a composite part of an autodisplay technology translocating a fluorescent protein (mCherry). This part includes an T7 promoter with a lacI operator as well as an RBS(AGGA). The fluorescent protein will cleave post-translationally. This part can be used to quantify the surface density and activity of autodisplay technology. PET is a beta-barrel autodisplay technology native to E.coli. mCherry is a derivative of RFP.Notes
To comply with BioBrick standard 10, Phe-76 was re-optimized to remove a EcoRI site within the part. A T7 promoter with a lacI operator was added with the RBS (AGGA) to the PET_mCherry. A standard assembly 10 prefix and suffix was then added.Source
A sequence optimized for E. coli for a PET beta barrel auto-transporter expressing mCherry was obtained from Sevastynovich et al."A generalised module for the selective extracellular accumulation of recombinant proteins." Microbial Cell Factories. 2012. The sequence was assembled from synthesized 500bp blocks through IDTdna "Gblock" synthesis.| Sequence Annotation | Location | Component / Role(s) |
| Signal Peptide mCherry Autochaperone Translocator Domain Start Stop RBS | 119,274 317,1024 1235,1546 1547,2458 119,121 2459,2466 9,12 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc start_codon feature/start feature/stop stop_codon ribosome_entry_site feature/rbs |