Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: TU Munich 2013
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-28 03:52:04
Secretory NanoLuc Luciferase (IgKappa-SigP_nLuc) in RFC[25] N-Part
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1159006_sequence (Version 1) |
Description
NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from Renilla reniformis or from Photinus pyralis (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa). This part encodes for a NanoLuc Luciferase which is fused to a N-terminal signal peptide for secretion (IgKappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.Notes
xSource
Synthetic| Access | Instance | Definition |
| public public public | BBa_K1159304 BBa_K1159993 BBa_K1159001 | BBa_K1159304 BBa_K1159993 BBa_K1159001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1159304 BBa_K1159993 BBa_K1159001 | 1,67 68,73 74,583 | BBa_K1159304 BBa_K1159993 BBa_K1159001 |