Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:31
PhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a light-switchable TEV-Protease) in RFC[25]
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1159106_sequence (Version 1) |
Description
This parts encodes are fusion protein consisting the photosensitive core of PhyB (2-908 AA) at the N-terminus and the C-terminal half of TEV Protease at its C-terminus. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions.Expressed with the second essential protein http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315 or http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315 containing the part which interacts with PhyB and the N-terminal half of the splitted TEV Protease, it form a light-switchable TEV Protease which is activated by red light and inactivated by far-red light.
Note: PhyB requires Phycocyanobilin or Phytochromobilin as a cofactor to fulfill its function as photoswitchable protein; you can reach it by adding exogenous Phycocyanobilin/Phytochromobilin in the medium or by inserting genes for the Phycocyanobilin/Phytochromobilin synthesis. See here for more information: http://2012.igem.org/Team:TU_Munich/Project/Light_Switchable_Promoter#Biosynthesis_of_Phycocyanobilin
Notes
xSource
Synthetic| Access | Instance | Definition |
| public public public public public | BBa_K801031 BBa_K1159993 BBa_K243029 BBa_K1159993 BBa_K1159102 | BBa_K801031 BBa_K1159993 GSATLinker BBa_K1159993 BBa_K1159102 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K801031 BBa_K1159993 BBa_K243029 BBa_K1159993 BBa_K1159102 | 1,2721 2722,2727 2728,2835 2836,2841 2842,3195 | BBa_K801031 BBa_K1159993 GSATLinker BBa_K1159993 BBa_K1159102 |