Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:31
FRET Reporter for TEV Protease activity (eCFP_TEV-site-linker_SYFP2) in RFC[25]
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1159112_sequence (Version 1) |
Description
This part encodes a FRET reporter with the chromophores eCFP and SYFP2 that is seperated by a TEV cleavage site. As a FRET pair, excitation of eCFP leads to a energy transfer from eCFP to SYFP2 (FRET) resulting in a fluorescence of SYFP2. In the presence of TEV Protease cleavage actitivy the FRET pair SYFP2 is cleaved off from eCFP and eCFP is not anymore quenched by SYFP2 resulting in the fluorescence of eCFP. This part is flanked by RFC[25] pre- and suffix for further protein fusions.Notes
xSource
Synthetic| Access | Instance | Definition |
| public public public public public | BBa_K1159302 BBa_K1159993 BBa_K1159318 BBa_K1159993 BBa_K1159301 | BBa_K1159302 BBa_K1159993 BBa_K1159318 BBa_K1159993 BBa_K1159301 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1159302 BBa_K1159993 BBa_K1159318 BBa_K1159993 BBa_K1159301 | 1,714 715,720 721,747 748,753 754,1467 | BBa_K1159302 BBa_K1159993 BBa_K1159318 BBa_K1159993 BBa_K1159301 |