Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:31
Membrane-anchored SpyTag with secretory nLuc_SpyCatcher reporter fusion translational unit
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1159214_sequence (Version 1) |
Description
This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyCatcher and a secretory nLuc_SpyTag (IgKappa-SigP_nLuc_SpyTag) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized catcher protein and the secretory fusion protein containing N-terminal nLuc and C-terminal SpyTag Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.Notes
xSource
Synthetic| Access | Instance | Definition |
| public public public public public | BBa_K1159207 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159210 | BBa_K1159207 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159210 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1159207 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159210 | 1,1147 1148,1164 1165,1791 1792,1797 1798,2725 | BBa_K1159207 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159210 |