BBa_K1159316

BBa_K1159316 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1159316

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: TU Munich 2013

Date created: 2013-09-15 11:00:00

Date modified: 2015-06-11 01:53:10

SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1159316_sequence (Version 1)

Description

This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing Strep-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Note: A signal peptide is needed for membrane integration.

Notes

x

Source

Synthetic

Components

Access	Instance	Definition
public	BBa_K1159309	BBa_K1159309
public	BBa_K1159993	BBa_K1159993
public	BBa_K1159315	BBa_K1159315

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1159309	1,57	BBa_K1159309
BBa_K1159993	58,63	BBa_K1159993
BBa_K1159315	64,996	BBa_K1159315

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1159316/1

Member of these Collections

• //chassis/eukaryote/ppatens
• iGEM Parts Registry