Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yang Rongwu
Date created: 2013-09-13 11:00:00
Date modified: 2015-05-08 01:09:32
chimeric RNA，
combining crRNA and tracrRNA
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1160001_sequence (Version 1) |
Description
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system provide protection against mobile genetic element by guided specific DNA cleavage. Cas9 protein belongs type II CRISPR system and showed strong DNA cleavage activity. In order to accomplish site specific cleavage, cas9 protein must be guided by crRNA and tracrRNA. This plasmid carries the fragment corresponding to a chimeric RNA that combines crRNA and tracrRNA. The crRNA is designed to target part BBa_K1160002, which is used to examine the DNA cleavage conducted by Cas9 protein or detect the existence of CRISPR system in other organism. The crRNA segment is replaceable，and it's flanked by BsaA I site. (both upstream and down stream)You can treat this part with BasA I and ligate it with your seed sequence (your seed sequence must also be flanked by BasA I sticky end).
NOTE：crRNA contains the sequence that is complementary to your target sequence.
Notes
to be added.Source
The DNA sequence corresponding to crRNA is from the type I CRISPR system in E.coli stri. k12, substr. W3110. (NCBI AC number: NC_007779).The DNA sequnce corresponding to tracrRNA is Streptococcus pyogenes.
| Sequence Annotation | Location | Component / Role(s) |
| Spacers and Repeats hPGK promoter BSD SV40 poly(A) ori AmpR AmpR promoter | 4051,4813 456,966 978,1376 1466,1587 2006,2594 2765,3625 3626,3730 | feature/misc sequence_feature promoter feature/promoter CDS feature/protein sequence_feature feature/misc feature/misc sequence_feature CDS feature/protein feature/promoter promoter |