BBa_K1162014

BBa_K1162014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1162014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ryan Putman

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:09:32

protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1162014_sequence (Version 1)

Description

N-terminal 10x His-Tag fused with GFP (no translational stop codon) to allow for N-terminal HT-GFP tagged protein fusion. RFC 23 allows for in frame fusion on the C-terminal end of GFP.

Notes

RFC 23. ATG(Met) at start on coding region.

Source

GFP is synthetic form of GFP from a Jellyfish (Aequorea victoria).

Components

Access	Instance	Definition
public	BBa_K1162010	BBa_K1162010
public	BBa_K1162011	BBa_K1162011

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1162010	1,259	BBa_K1162010
BBa_K1162011	266,982	BBa_K1162011

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1162014/1

Member of these Collections

• iGEM Parts Registry