BBa_K1163101

BBa_K1163101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1163101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Emiel van der Kouwe

Date created: 2013-09-21 11:00:00

Date modified: 2015-08-03 03:10:42

pAceB promoter region

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1163101_sequence (Version 1)

Description

Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.

Why would you use this part?

This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the Fes promoter region decreases the expression of the downstream gene.

Notes

We blindly extracted the 300 bp sequence upstream of the AceB gene in E. coli. It contains a putative FUR binding site and a putative RBS.

Source

Genomic DNA.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pAceB	1,300	feature/promoter, promoter

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1163101/1

Member of these Collections

• //function/sensor/metal
• iGEM Parts Registry