BBa_K1163102

BBa_K1163102 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1163102

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Emiel van der Kouwe

Date created: 2013-09-21 11:00:00

Date modified: 2015-05-08 01:09:32

pAceB-sfGFP-Term

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1163102_sequence (Version 1)

Description

Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.

Why would you use this part?

This part allows the characterization of the AceB promoter region thanks to the sfGFP downstream.

Notes

None

Source

Golden Gate assembly between BBa_K1163101 and BBa_K1163002

Components

Access	Instance	Definition
public	BBa_K1163101	BBa_K1163101
public	BBa_K1163002	BBa_K1163002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1163101	1,300	BBa_K1163101
BBa_K1163002	307,1163	BBa_K1163002

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1163102/1

Member of these Collections

• iGEM Parts Registry