BBa_K1163104

BBa_K1163104 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1163104

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Emiel van der Kouwe

Date created: 2013-09-21 11:00:00

Date modified: 2015-05-08 01:09:32

FepA promoter region

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1163104_sequence (Version 1)

Description

Promoter from E. coli that controls the FepA gene, involved in iron uptake and iron sensitive.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.


Why would you use this part?


This promoter allows you to have an iron-sensing behaviour. In fact, in the presence of iron, the FepA promoter region decreases the expression of the downstream gene.

Notes

We blindly extracted the 320 bp sequence upstream of the FepA gene in E. coli. It contains a putative FUR binding site and a putative RBS.

Source

Genomic sequence

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
FepA	1,320	promoter, feature/promoter

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1163104/1

Member of these Collections

• iGEM Parts Registry