BBa_K1163400

BBa_K1163400 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1163400
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emiel van der Kouwe
Date created: 2013-09-21 11:00:00
Date modified: 2015-05-08 01:09:32

AceB promoter, iron sensitive, down-regulated by dimerized FUR protein



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1163400_sequence (Version 1)

Description

Promoter from E. coli that controls the AceB gene, involved in iron uptake.

This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence or iron in the range 10^-7, 10^-5 mol.L-1

Notes

Impossible to idengitfy the RBS and FUR binding site precisely

Source

Extracted form genomic sequence of E. coli MG1655

Sequence Annotation Location Component / Role(s)
Putative shine-delgarno sequence
285,294
ribosome_entry_site feature/rbs
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1163400/1