BBa_K1164002

BBa_K1164002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1164002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wilson Lam, Dylan Siriwardena

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:09:32

LacI repressor tagged with yBFP

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1164002_sequence (Version 1)

Description

This is the LacI repressor from E.coli tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm. It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

This is an updated version of BBa_K642001 that is functional in S. cerevisiae.

Notes

In order to remove the illegal XbaI site, site-directed mutagenesis was performed, changing nucleotide 255 from a thymine to a cytosine, and also changing nucleotide 258 from an adenine to a glycine.

Source

E.coli/commercial synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacI	1,1080	CDS, feature/cds
yBFP	1081,1782	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1164002/1

Member of these Collections

• iGEM Parts Registry