BBa_K1164008

BBa_K1164008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1164008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wilson Lam, Dylan Siriwardena, Lloyd Mai, Yara Abou-Hamde

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:09:32

pTRELX driving yeGFP with tPGK1 terminator

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1164008_sequence (Version 1)

Description

This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. In addition, two lacO operators have been introduced downstream of the TATA box, allowing for repression of gene expression by lacI. Upon expression from the promoter, yEGFP will be produced.

Notes

N/A

Source

S.cerevisiae/commercial synthesis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
tetO	7,25	operator, feature/operator
tetO	28,46	operator, feature/operator
tetO	47,65	operator, feature/operator
tetO	113,131	feature/operator, operator
lacO	352,368	feature/operator, operator
pTRELX	1,558	feature/promoter, promoter
yEGFP	559,1272	CDS, feature/cds
tPGK1	1273,1466	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1164008/1

Member of these Collections

• iGEM Parts Registry