BBa_K1166003

BBa_K1166003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1166003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luis Mario Leal Garza
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:33

His-TAT



Types
DnaRegion

Roles
engineered_region

Signalling

Sequences BBa_K1166003_sequence (Version 1)

Description

A biobrick that includes the TAT signal peptide that can be fused to any protein for an easy internalization into mammal cells. It includes a 6x His-tag for easy purification.
TAT is a cell penetrating peptide (CPP) derived from the transactivator of transcription from HIV and has been used to internalize a range of large and small molecules from peptides, DNA and antibodies, to liposomes.

Notes

We include a 6x Histidine tag for purification. TAT peptide is ready to be fused at N-terminal region.

Source

We took the sequences needed for these part from a sequence alignment.

Sequence Annotation Location Component / Role(s)
start
His-tag
linker
TAT
linker
1,3
4,21
22,51
52,84
85,114
feature/start start_codon
feature/tag tag
sequence_feature feature/misc
CDS feature/cds
feature/misc sequence_feature
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1166003/1