Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:33
TAT-Apoptin
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1166005_sequence (Version 1) |
Description
Codes for the T7 expression cassette for Apoptin composed of a N-terminal TAT translocation peptide, followed by a linker (GGGGS), a histidine tag (6x) and by the same linker fused to Apoptin. Apoptin is a protein from the Chicken Anemia Virus known to cause apoptosis in human cancer cell lines only using a pathway independent from P53 by a phosphorilation in the Threonine 108.Notes
A histidine tag was used for purification and TAT peptide was included for the toxin to penetrate mammalian cells.Source
This part was synthesized by Genscript.| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_B0034 spacer His tag start linker TAT linker Apoptin | 1,46 47,58 59,64 68,85 65,67 86,115 116,148 149,178 179,676 | promoter feature/promoter ribosome_entry_site feature/rbs sequence_feature feature/misc tag feature/tag start_codon feature/start feature/misc sequence_feature feature/cds CDS sequence_feature feature/misc CDS feature/cds |