Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas K. Goldner
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:35
endo-beta-1,3-1,4 glucanase (BglS) from Bacillus Subtilis 168
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1175006_sequence (Version 1) |
Description
BglSThis gene encodes an endo-beta-1,3-1,4-glucanase (bglS), which is from the bacterium Bacillus subtilis subtilis 168. The enzyme will hydrolyze and thereby cleave internal 1,4 linkages adjacent to 1,3 linkages.
Usage and Biology
The substrates vulnerable to the bglS encoded enzyme are mixed linked beta-glucans. These glucans would have 1,3 and 1,4 beta linkages within the polysaccharide linking together the glucose monomers. Examples of these glucans can be found in oats, maize, and barley.
(1) http://mic.sgmjournals.org/content/141/2/281.long
(2) http://onlinelibrary.wiley.com/doi/10.1002/j.2050-0416.1984.tb04259.x/pdf
Notes
No design constraints were needed for RF10 insertion, a second stop codon was also added to the end of the coding region.Source
Bacillus Subtilis 168| Sequence Annotation | Location | Component / Role(s) |
| Start Codon coding region Double Stop Codon | 1,3 4,726 727,732 | feature/start start_codon feature/protein CDS feature/stop stop_codon |