Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas K. Goldner
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:35
Strong pBAD promoter BBa_K206000 attached to SecI pump BBa_K215104
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1175028_sequence (Version 1) |
Description
These are the three essential parts prtD, prtE, prtF, that make up the Type I secretion system from Erwinia chrysanthemi.This part is made of 3 genes: prtD, prtE, and prtF, that constitute a type I Erwinia chrysanthemi secretion system. The Pump is expressed from a strong constitutive promoter, BBa_k206000, and has the translational terminator BBa_B0014. In pSB1C3.Used to secrete proteins containing prtB C-terminal tag. The prtB C-terminal tag is built into the protein generator [BBa_K215002]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.
For full characterization data on this part, please see the University of Washington 2009 iGEM project page and Wisconsin Lutheran College 2013 iGEM project page.
Notes
We had to remove the RBS and in the future will be removing all Bative RBSs from the sequence to be replaced with BBa_B0034Source
iGEM registry, acquired from University of Washington 2009 iGEM team from the genomic DNA of Erwinia chrysanthemi| Access | Instance | Definition |
| public public | BBa_K206000 BBa_K215104 | pBAD BBa_K215104 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_K215104 | 1,130 139,4668 | pBAD BBa_K215104 |