Types | DnaRegion
|
Roles | promoter
Regulatory
|
Sequences | BBa_K206000_sequence (Version 1)
|
Description
pBAD is an
E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (
I13453) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
Notes
There were no special design considerations.
Source
The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].