pBAD

BBa_K206000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K206000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amelia Hardjasa
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:11:23

pBAD strong



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K206000_sequence (Version 1)

Description

pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (I13453) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.

Notes

There were no special design considerations.

Source

The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].

Sequence Annotation Location Component / Role(s)
promoter
AraI1
AraI2
1,131
40,57
61,78
promoter feature/promoter
feature/operator operator
feature/operator operator
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K206000/1