BBa_K1179008

BBa_K1179008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1179008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nelson Hall

Date created: 2013-09-14 11:00:00

Date modified: 2015-05-08 01:09:35

A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1179008_sequence (Version 1)

Description

This plasmid contains the coding sequence for a DNA binding non-nuclease mutant of Cas9 followed by a linker sequence and a BsaI site for ligation of a protein of interest to the C-terminal end of the Cas9. When expressed with a guide RNA, the Cas9 will target the location as dictated by the guide RNA and localize your fused protein to that region.

Notes

Design

Source

Source

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1179008/1

Member of these Collections

• iGEM Parts Registry