BBa_K1179017

BBa_K1179017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1179017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nelson Hall
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:35

A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1179017_sequence (Version 1)

    Description

    This plasmid contains the coding sequence for a DNA binding non-nuclease mutant of Cas9 preceded by a linker sequence and a BsaI site for ligation of a protein of interest to the C-terminal end of the Cas9. When expressed with a guide RNA, the Cas9 will target the location as dictated by the guide RNA and localize your fused protein to that region.

    Notes

    Design

    Source

    Source

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1179017/1
     

    Attachments

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